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KMID : 0385520080210060502
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Analytical Science & Technology
2008 Volume.21 No. 6 p.502 ~ p.509
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Quantification of cholesterol in human serum by isotope dilution liquid chromatography/mass spectrometry
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Shin Hye-Sun
Lee Hwa-Shim
Lee Gae-Ho
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Abstract
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An ID LC/MS (isotope dilution liquid chromatography/mass spectrometry) was used as a primary method for the quantitative analysis of cholesterol in human serum. The separation of cholesterol was carried out by Thermo ODS hypersil C18 column. The mobile phase was 100% methanol, and flow rate was 0.3 ㎖/min. Cholesterol and cholesterol-3,4-13C₂ were monitored at m/z 369.4 and 371.3, which correspond to [M-H₂O+H]? respectively. In order to verify the measurement method, NIST SRM 909b was analyzed. The results agreed well with certified values within uncertainty. The four kinds of serum certified reference material were prepared and certified. The repeatabilities of measurement were ranged from 0.1 to 0.8% (RSD), which were relatively good. The reproducibility between independent measurement run was below 0.24% (RSD). The expanded uncertainty was about 1.43% within the 95% confidence interval.
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KEYWORD
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cholesterol, ID LC/MS, primary method, traceability
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